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3T3 Neutral Red Uptake Phototoxicity Assay Applications


The 3T3 NRU phototoxicity assay is generally used to test the phototoxic potential of chemicals and ingredients, rather than final products and formulations.

Test Material Compatibility

The assay is best suited for water-soluble materials; however, test materials may be dissolved in intermediate solvents so that they are compatible with the test system. At IIVS, an initial solubility test is performed to find the most suitable solvent when the solubility of a test chemical is unknown.

Test materials that are found to be insoluble or otherwise incompatible with this test system (3T3 NRU Phototox) may be tested using our 3-D Reconstructed Human Epidermis Phototoxicity assay.

Specialized Protocols

Test article concentrations may be adjusted as necessary to accommodate specific physical characteristics or client needs. Specialized protocols may be prepared as requested through consultation with the Study Director.

Alternate assays measuring the potential for phototoxic action may be employed using 3-dimensional reconstructed human epidermal tissue models. Please contact us for more information.

Validation Status

OECD TG432

Phototoxicity Comparisons