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The predictive capacity of the 3T3 Neutral Red Uptake assay to identify substances LD with acute oral LD50 50 > kg> 2000 mg/kg poster presented at IUTOX
Published: August 4th, 2010 | Category: Whats New
The purpose of this validation study is to assess the capacity of the 3T3/NRU cytotoxicity assay to give a simple yes or no answer in order to discriminate between toxic/hazardous (LD50 < 2000 mg/kg) and not classified (LD50 >2000 mg/kg) substances....
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The predictive capacity of the 3T3 Neutral Red Uptake assay to identify substances LD with acute oral LD50 50 > kg> 2000 mg/kg
Published: March 8th, 2010 | Category: IIVS Publications
Source: SOT
The purpose of this validation study is to assess the capacity of the 3T3/NRU cytotoxicity assay to give a simple yes/no answer in order to discriminate between toxic/hazardous (LD50 < 2000 mg/kg) and not classified (LD50 >2000 mg/kg) substances. High (87%) prevalence of substances with acute oral LD50 > 2000 mg/kg. Registry of Cytotoxicity showed ...
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The EpiOcular model – protocol, performance and experience
Published: August 26th, 2005 | Category: IIVS Publications
Source: Practical Training Workshop on In Vitro Eye Irritation Methods, August 26, 2006, Berlin, Germany
Practical Training Workshop on In Vitro Eye Irritation Methods
Co-sponsored by:
Institute for In Vitro Sciences, IIVS (Gaithersburg, USA)
Beiersdorf AG (Hamburg, Germany)
BfR-ZEBET (Berlin, Germany)
SCOPE: The Workshop aims at practical training in most frequently used in vitro methods for
prediction of eye irritation potential. Primary focus...
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The BCOP – protocol, performance and experience
Published: August 26th, 2005 | Category: IIVS Publications
Source: Practical Training Workshop on In Vitro Eye Irritation Methods, August 26, 2005,Berlin, Germany
Practical Training Workshop on In Vitro Eye Irritation Methods
Co-sponsored by:
Institute for In Vitro Sciences, IIVS (Gaithersburg, USA),
Beiersdorf AG (Hamburg, Germany),
BfR-ZEBET (Berlin, Germany).
SCOPE: The Workshop aims at practical training in most frequently used in vitro methods for
prediction of eye irritation potential. Primar...
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EPIOCULAR™ HUMAN CELL CONSTRUCT: TISSUE VIABILITY AND HISTOLOGICAL CHANGES FOLLOWING EXPOSURE TO SURFACTANTS
Published: August 21st, 2005 | Category: IIVS Publications
Source: Presented at the 5th World Congress on Alternatives and Animal Use in the Life Sciences, Berlin, Germany, August 21-26, 2005
The ability of the EpiOcularTM construct to predict the eye irritation potential of surfactants has been the
subject of a formal validation program. EpiOcularTM correlates a test article's potential for ocular irritation
with the time it takes to reduce tissue viability by 50% (ET(sub>50) as measured by MTT. This study ...
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OPTIMIZATION OF AN IN VITRO LONG TERM CORNEAL CULTURE ASSAY
Published: August 21st, 2005 | Category: IIVS Publications
Source: Presented at the 5th World Congress on Alternatives and Animal Use in the Life Sciences, Berlin, Germany, August 21-26, 2005
The long-term culture of corneas has been proposed as an in vitro model to evaluate potential eye irritation and post-treatment recovery following chemical exposures. Porcine eyes were btained within 24 hours of sacrifice and disinfected prior to excising corneas. Corneas were filled with an agar/gelatin gel in M199
medium to support the corneas, ...
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PERFORMANCE STANDARDS FOR IN VITRO STUDIES
Published: August 21st, 2005 | Category: IIVS Publications
Source: Presented at the 5th World Congress on Alternatives and Animal Use in the Life Sciences, Berlin, Germany, August 21-26, 2005
In vitro test systems offer the potential for high consistency and resulting high predictive capacity. This promise comes from the ability to control many of the independent variables that can lead to the variability of responses in vivo. Furthermore, most of the in vitro tests currently employed, have undergone some level of formal validation or a...
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Addressing Animal Testing Concerns: A Novel Micronucleus Assay Using The Human 3-D Skin Model, EpiDerm™
Published: August 21st, 2005 | Category: IIVS Publications
Source: Presented at the 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin Germany, Aug. 21-26, 2005
To meet the requirements of the EU 7th Amendment to the Cosmetics Directive, manufacturers of cosmetics
products will need to ascertain the safety of ingredients using non-animal methods. Starting in 2009, in vivo
genotoxicity tests for cosmetic ingredients will not be allowed. Skin is one of the target areas of interest for
many cosmetic produc...
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Applying Good Laboratory Practices (GLPs) to In Vitro Studies: One Laboratory's Perspective
Published: August 21st, 2005 | Category: IIVS Publications
Source: Presented at the 5th World Congress on Alternatives and Animal Use in the Life Sciences, Berlin, Germany, August 21-26, 2005
The steady increase in industry use and regulatory acceptance of in vitro test methods has resulted in an increased need to apply Good Laboratory Practice (GLP) regulations to these systems. The original GLP regulations, developed to address the conduct of animal studies, are concerned with many special conditions that apply to animal housing and c...
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EPIOCULAR™ HUMAN CELL CONSTRUCT: TISSUE VIABILITY AND HISTOLOGICAL CHANGES FOLLOWING EXPOSURE TO SURFACTANTS
Published: March 6th, 2005 | Category: IIVS Publications
Source: Presented at the 44th Annual Meeting of the Society of Toxicology, New Orleans, Louisiana, March 6-10, 2005
The ability of the EpiOcular™ construct to predict the eye irritation potential of surfactants and surfactant-based formulations has been the subject of a formal validation program. EpiOcular™ correlates a test article's potential for ocular irritation with the time it takes to reduce tissue viability by 50% (ET50) as measured by the tis...
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MANAGING TOXIC SYNERGISM IN HYPOCHLORITE-CONTAINING CLEANERS USING THE BOVINE CORNEAL OPACITY AND PERMEABILITY (BCOP) ASSAY, Part II
Published: March 12th, 2004 | Category: IIVS Publications
Consumers continue to seek products that will both clean and combat mold and mildew stains on various surfaces in the home. Such products are commonly formulated as alkaline hypochlorite and surfactant-containing aqueous solutions and usually have
significant ocular irritation potential. Previous studies have shown the applicability of the BCOP As...
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ASSESSMENT OF PROTOCOL VARIABLES IN CYTOTOXICITY ASSAYS UTILIZING BALB/C 3T3 CELLS AND NORMAL HUMAN KERATINOYTES
Published: March 1st, 2003 | Category: IIVS Publications
Source: Presented at the Society of Toxicology Annual Meeting, 2003
The NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) and the European Center for the Evaluation of Alternative Methods (ECVAM) are currently sponsoring a study to determine the usefulness of two in vitro basal cytotoxicity methods [employing BALB/c 3T3 (3T3) cells and normal human keratinocytes (NHK)] for pre...
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A prevalidation study on in vitro tests for acute skin irritation: results and evaluation by the Management Team
Published: January 1st, 2001 | Category: IIVS Publications
Source: Reprinted from Toxicology In Vitro, Vol. 15, pp. 57-93, 2001, with permission from Elsevier Science (ScienceDirect TM http://www.sciencedirect.com)
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Prediction of ocular irritancy of prototype shampoo formulations by the isolated rabbit eye (IRE) test and bovine corneal opacity and permeability (BCOP) assay.
Published: January 1st, 2001 | Category: IIVS Publications
Source: Reprinted from Toxicology In Vitro, Vol. 15, p. 95-103, 2001, with permission from elsevier Science. (ScienceDirectTM http://www. sciencedirect.com)
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13th Meeting of the Scientific Group on Methodologies for the Safety Evaluation of Chemical (SGOMSEC): Validation and Acute Testing
Published: April 1st, 1998 | Category: IIVS Publications
Source: Environmen Health Perspect 106 (suppl 2):419-425 (1998)
Scientific principles demand that before newly developed alternative methods for safety testing are fully embraced by the regulatory community, they reliably and reproducibly predict the designated toxic end point. The process used to determine reliability and reproducibility is termed validation, and it generally culminates with a highly controlle...
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The ECVAM International Validation Study on In Vitro Tests for Skin corrosivity. 2. Results and Evaluation by the Management Team
Published: January 1st, 1998 | Category: IIVS Publications
Source: Reprinted from Toxicology In Vitro, Vol. 12, pp. 483-524, 1998, with permission from Elsevier Science (ScienceDirect TM http://www.sciencedirect.com)
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Evaluation of the Human Epidermal Keratinocyte Neutral Red Uptake Assay Using the First 10 MEIC Test Materials
Published: January 1st, 1992 | Category: IIVS Publications
Source: Reprinted from Toxicology In Vitro, vol. 6, No. 4, pp.367-371, 1992, with permision from Elsevier Science. (ScienceDirectTM http://www.scicencedirect.com)
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