Evaluation Of A Novel Micronucleus Assay Using A Human 3-D Skin Model, Epiderm™
Published: August 22nd, 2006
by Curren, Rodger D.; Aardema, Marilyn J.; Hayden, Patrick J.; Mun, Greg; Hu, Ting; Wilt, Nathan; Gibson, Dave SOURCE
Presented at the Society of Toxicology Annual Meeting, 2006
SUMMARY
For many chemicals and products, most notably cosmetics, skin is the highest exposed tissue; however, very few assays are available to directly address potential genotoxicity to this tissue. Although rodent models are being developed to measure micronucleus induction in the skin, European legislation such as the 7th amendment to the Cosmetics Directive precludes the use of in vivo assays for genotoxicity assessments of cosmetic ingredients after 2009. To provide a useful alternative, we are developing an in vitro human skin micronucleus assay using the 3-D EpiDermTM skin model (MatTek Corp, Ashland, MA). Theoretically such a model could approximate the complexities typical of in vivo exposures, e.g. absorption, tissue specificity, metabolism, etc., and at the same time reflect human-specific responses in these parameters.
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