EURL ECVAM Releases Recommendations on the Direct Peptide Reactivity Assay (DPRA) for Skin Sensitization Testing
EURL ECVAM has fully endorsed the ECVAM Scientific Advisory Committee's (ESAC) opinion on the ECVAM coordinated validation study of the Direct Peptide Reactivity Assay (DPRA). They have found that information from peptide reactivity assays, such as the DPRA, is relevant for the assessment of the skin sensitization potential of chemicals. The DPRA is transferable to suitably equipped laboratories that are proficient in high performance liquid chromatography (HPLC) analysis, and both the within and between laboratory repeatability was quite high (87% and 75% respectively). Although full review of the assay's predictive capacity was outside the scope of this review, its accuracy of distinguishing between sensitizers and non-sensitizers is 82%. To read EURL ECVAMs' full recommendation, please follow the link above.