FutureTox will address the challenges and opportunities associated with effective and efficient implementation of the explosion of 21st century toxicity testing technologies and tools into improved, science-informed hazard prediction and risk assessment. There is a common desire to use 21st century tools and approaches in hazard identification, and risk assessment. As of yet no one roadmap exists for coalescing disparate approaches into a consistent and coherent strategy. The workshop will have four major themes, starting with a review and discussion of the various strategies being considered as potential roads to the desired destination. This opening session will promote identification of common themes and key considerations and requirements essential to an ordered and rational implementation of the road design. The following three session themes are intended to stimulate more detailed dialog and understanding of “what, when and how” considerations whereby newly emerging technologies can be most constructively engaged in transitioning to the future vision. The themes will discuss (a) Toxicology testing in the 21st Century (TT21c) approaches for safety assessments and expectations for effective and efficient integration into and potential transition of existing safety assessment practices (b) TT21c approaches for exposure assessments and links to toxicity test dosimetry, with a particular focus on relationships to contextual dose-exposure considerations associated with high throughput in vitro evaluation systems; and finally (c) Reframing risk assessment practices, with an emphasis on how emerging science can best impact and reshape current risk assessment practice.